Molecular cloning of CLC chloride channels in Oreochromis mossambicus and their functional complementation of yeast CLC gene mutant.

@article{Miyazaki1999MolecularCO,
  title={Molecular cloning of CLC chloride channels in Oreochromis mossambicus and their functional complementation of yeast CLC gene mutant.},
  author={Hiroaki Miyazaki and Shoko Uchida and Yoshifumi Takei and Toshihiko Hirano and Fumiaki Marumo and Shogo Sasaki},
  journal={Biochemical and biophysical research communications},
  year={1999},
  volume={255 1},
  pages={
          175-81
        }
}
We have cloned two members of the CLC chloride channel family (OmCLC-3 and OmCLC-5) from gill cDNA libraries of the euryhaline tilapia Oreochromis mosammbicus. At the amino acid level, OmCLC-3 is 90.5% identical to rat CLC-3 and OmCLC-5 is 79.2% identical to rat CLC-5. Ribonuclease protection assay revealed that OmCLC-5 was mainly expressed in the gill, kidney, and intestine in both freshwater- (FW) and seawater- (SW) adapted tilapia. Although the mRNA of OmCLC-3 was broadly expressed in… CONTINUE READING

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