Molecular cloning of Brassica rapa nitrilases and their expression during clubroot development.

@article{Ishikawa2007MolecularCO,
  title={Molecular cloning of Brassica rapa nitrilases and their expression during clubroot development.},
  author={Toshiki Ishikawa and Keiichi Okazaki and Haruka Kuroda and Kimiko Itoh and Toshiaki Mitsui and Hidetaka Hori},
  journal={Molecular plant pathology},
  year={2007},
  volume={8 5},
  pages={623-37}
}
SUMMARY Three isoforms of nitrilase were cloned from turnip, Brassica rapa L., and their expression during clubroot development caused by Plasmodiophora brassicae was investigated. The isoforms were designated BrNIT-T1, BrNIT-T2 and BrNIT-T4 based on homology to known nitrilases. BrNIT-T1 and BrNIT-T2 have 80% homology to three nitrilases from Arabidopsis thaliana (AtNIT1, AtNIT2 and AtNIT3). BrNIT-T4 showed 90% homology to AtNIT4. To confirm their enzyme activity, the recombinant proteins were… CONTINUE READING