Molecular cloning of Ancient Egyptian mummy DNA

  title={Molecular cloning of Ancient Egyptian mummy DNA},
  author={Svante P{\"a}{\"a}bo},
Artificial mummification was practised in Egypt from ∼ 2600 BC until the fourth century AD. Because of the dry Egyptian climate, however, there are also many natural mummies preserved from earlier as well as later times. To elucidate whether this unique source of ancient human remains can be used for molecular genetic analyses, 23 mummies were investigated for DNA content. One 2,400-yr-old mummy of a child was found to contain DNA that could be molecularly cloned in a plasmid vector. I report… 

Genetic analyses from ancient DNA.

The precautions and criteria necessary to ascertain to the greatest extent possible that results represent authentic ancient DNA sequences are discussed, which highlight some significant results and areas of promising future research.

DNA decay rate in papyri and human remains from Egyptian archaeological sites.

The DNA half-life in papyri is about 19-24 years, which means that the last DNA fragments will vanish within no more than 532-672 years from the sheets being manufactured, an indirect argument against the reliability of claims about the recovery of authentic DNA from Egyptian mummies and bone remains.

Paleogenetic Study of Ancient Mummies at the Kurchatov Institute

The article presents the results of experimental studies that determined the mitochondrial and Y-chromosomal haplogroups of some of the studied mummies, the sex previously established by anthropologists was confirmed, the DNA of certain pathogens were found, and isotope analysis was carried out.

Genetic Analyses of Bovid Remains and the Origin of Early European Cattle

The results presented here show that cattle domestication was more complicated than previously suggested and it appears therefore that today’s cattle descend both from both Anatolian and European aurochsen.

Kopalny DNA czyli lekcja z przeszłości

DNA sequencing of the entire mitochondrial cytochrome b of Myotis myotis sensu lato in the molecular laboratory allowed the comparison of aDNA sequences (dating back to ca. 830 years BP) with those of modern bats to assess their genetic relationships.

Analysis of ancient Japanese society through mitochondrial DNA sequencing

The results of this study show that intensive analysis of ancient DNA from archaeological sites is a useful tool for investigating the social systems of vanished populations and strengthen the opinion that social differentiation began during this period in Japan.

Insights from ancient DNA analysis of Egyptian human mummies: clues to disease and kinship.

The current review tries to recapitulate the published studies presenting paleogenomic evidence of disease diagnosis and kinship establishment for the Egyptian human remains to make significant contributions to biomedical sciences and evidence-based archeology.

Ancient DNA: Using molecular biology to explore the past

  • T. A. BrownK. A. Brown
  • Biology
    BioEssays : news and reviews in molecular, cellular and developmental biology
  • 1994
Ancient DNA has been discovered in many types of preserved biological material, including bones, mummies, museum skins, insects in amber and plant fossils, and has become an important research tool

Authenticating Ancient Human Mitochondrial DNA

This work reports the use of a strategy to authenticate the identity of ancient mitochondrial DNA (mtDNA), based on the previously established relationship between D-loop sequence substitutions and haplogroup-specific restriction site changes.

Molecular anthropology: Touching the past through ancient DNA retrieval. Methodological aspects

  • H. Witas
  • Biology
    Anthropological Review
  • 2001
The revolution which introduced new techniques of molecular biology applied to DNA analysis enormously accelerated the progress in most areas of medicine and biology. Techniques such as polymerase



DNA sequences from the quagga, an extinct member of the horse family

Dried muscle from a museum specimen of the quagga, a zebra-like species that became extinct in 1883, is examined, and DNA was extracted in amounts approaching 1% of that expected from fresh muscle, and that the DNA was of relatively low molecular weight.

The Use of Natron in Mummification

Despite the numerous descriptions of the mode of embalming practised by the ancient Egyptians that have been written, there is still a considerable difference of opinion regarding both the method and the materials employed.

The histological examination of mummified material.

Tissue from Egyptian mummy material is extremely brittle; hence it was handled in perforated glass tubes during processing, which allowed regular sectioning and staining to be done successfully.

Filter replicas and permanent collections of recombinant DNA plasmids.

An improved, simple and inexpensive method for making paper filter replicas of such an ordered collection and of a high density (10,000 colonies/petri dish) unordered collection was developed, suitable for nucleic acid hybridization screens of recombinant DNA colinies.

The Alu family of dispersed repetitive sequences.

Property of this repeat sequence, its flanking sequences in chromosomal DNA, and RNA's transcribed from it suggest that it may be a mobile DNA element inserted at hundreds of thousands of different chromosomal locations.

Mummy of the "Elder Lady" in the tomb of Amenhotep II: Egyptian museum catalog number 61070.

An unidentified female mummy found in a cache of great kings and queens in 1898 in the Valley of the Kings was examined from the viewpoint of Egyptology, x-ray cephalometry, biostatistics, and