Molecular cloning of Ancient Egyptian mummy DNA

  title={Molecular cloning of Ancient Egyptian mummy DNA},
  author={S. P{\"a}{\"a}bo},
Artificial mummification was practised in Egypt from ∼ 2600 BC until the fourth century AD. Because of the dry Egyptian climate, however, there are also many natural mummies preserved from earlier as well as later times. To elucidate whether this unique source of ancient human remains can be used for molecular genetic analyses, 23 mummies were investigated for DNA content. One 2,400-yr-old mummy of a child was found to contain DNA that could be molecularly cloned in a plasmid vector. I report… Expand
Genetic analyses from ancient DNA.
The precautions and criteria necessary to ascertain to the greatest extent possible that results represent authentic ancient DNA sequences are discussed, which highlight some significant results and areas of promising future research. Expand
DNA decay rate in papyri and human remains from Egyptian archaeological sites.
The DNA half-life in papyri is about 19-24 years, which means that the last DNA fragments will vanish within no more than 532-672 years from the sheets being manufactured, an indirect argument against the reliability of claims about the recovery of authentic DNA from Egyptian mummies and bone remains. Expand
Paleogenetic Study of Ancient Mummies at the Kurchatov Institute
An interdisciplinary study of ten ancient Egyptian mummies from the collection of the Pushkin State Museum of Fine Arts, which date back to the 1st millennium BCE–beginning of the Common Era, wasExpand
Genetic Analyses of Bovid Remains and the Origin of Early European Cattle
The results presented here show that cattle domestication was more complicated than previously suggested and it appears therefore that today’s cattle descend both from both Anatolian and European aurochsen. Expand
Kopalny DNA czyli lekcja z przeszłości
DNA sequencing of the entire mitochondrial cytochrome b of Myotis myotis sensu lato in the molecular laboratory allowed the comparison of aDNA sequences (dating back to ca. 830 years BP) with those of modern bats to assess their genetic relationships. Expand
Analysis of ancient Japanese society through mitochondrial DNA sequencing
The results of this study show that intensive analysis of ancient DNA from archaeological sites is a useful tool for investigating the social systems of vanished populations and strengthen the opinion that social differentiation began during this period in Japan. Expand
Insights from ancient DNA analysis of Egyptian human mummies: clues to disease and kinship.
The current review tries to recapitulate the published studies presenting paleogenomic evidence of disease diagnosis and kinship establishment for the Egyptian human remains to make significant contributions to biomedical sciences and evidence-based archeology. Expand
Ancient DNA: Using molecular biology to explore the past
  • T. A. Brown, K. A. Brown
  • Biology, Medicine
  • BioEssays : news and reviews in molecular, cellular and developmental biology
  • 1994
Ancient DNA has been discovered in many types of preserved biological material, including bones, mummies, museum skins, insects in amber and plant fossils, and has become an important research toolExpand
Authenticating Ancient Human Mitochondrial DNA
This work reports the use of a strategy to authenticate the identity of ancient mitochondrial DNA (mtDNA), based on the previously established relationship between D-loop sequence substitutions and haplogroup-specific restriction site changes. Expand
Molecular anthropology: Touching the past through ancient DNA retrieval. Methodological aspects
The revolution which introduced new techniques of molecular biology applied to DNA analysis enormously accelerated the progress in most areas of medicine and biology. Techniques such as polymeraseExpand


DNA sequences from the quagga, an extinct member of the horse family
Dried muscle from a museum specimen of the quagga, a zebra-like species that became extinct in 1883, is examined, and DNA was extracted in amounts approaching 1% of that expected from fresh muscle, and that the DNA was of relatively low molecular weight. Expand
The Use of Natron in Mummification
Despite the numerous descriptions of the mode of embalming practised by the ancient Egyptians that have been written, beginning with that of Herodotus in the fifth century B . C . , there is still aExpand
The histological examination of mummified material.
Tissue from Egyptian mummy material is extremely brittle; hence it was handled in perforated glass tubes during processing, which allowed regular sectioning and staining to be done successfully. Expand
Base sequence studies of 300 nucleotide renatured repeated human DNA clones.
The nucleotide sequences of 15 clones constructed from these 300 nucleotide S 1 -resistant repeats are determined and ten of these cloned sequences are members of the Alu family of interspersed repeats, a dimeric structure that was evidently formed from a head to tail duplication of an ancestral monomeric sequence. Expand
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
Labeled DNAs (and restriction endonuclease fragments derived from them) are useful probes for detecting rare homologous sequences by in situ hybridization and reassociation kinetic analysis. Expand
Filter replicas and permanent collections of recombinant DNA plasmids.
An improved, simple and inexpensive method for making paper filter replicas of such an ordered collection and of a high density (10,000 colonies/petri dish) unordered collection was developed, suitable for nucleic acid hybridization screens of recombinant DNA colinies. Expand
The Alu family of dispersed repetitive sequences.
Property of this repeat sequence, its flanking sequences in chromosomal DNA, and RNA's transcribed from it suggest that it may be a mobile DNA element inserted at hundreds of thousands of different chromosomal locations. Expand
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
A series of plasmid vectors containing the multiple cloning site (MCS7) of M13mp7 has been constructed. In one of these vectors a kanamycin-resistance marker has been inserted into the center of theExpand
Mummy of the "Elder Lady" in the tomb of Amenhotep II: Egyptian museum catalog number 61070.
An unidentified female mummy found in a cache of great kings and queens in 1898 in the Valley of the Kings was examined from the viewpoint of Egyptology, x-ray cephalometry, biostatistics, andExpand
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
  • E. Southern
  • Biology, Medicine
  • Journal of molecular biology
  • 1975
This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters. The fragments can then be hybridized to radioactive RNA and hybrids detected byExpand