Molecular cloning of 3-hydroxy-3-methylglutaryl coenzyme a reductase and evidence for regulation of its mRNA.

@article{Chin1982MolecularCO,
  title={Molecular cloning of 3-hydroxy-3-methylglutaryl coenzyme a reductase and evidence for regulation of its mRNA.},
  author={Daniel J. Chin and Kenneth L. Luskey and Jerry R Faust and Raymond J MacDonald and Michael Scott Brown and Joseph L Goldstein},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1982},
  volume={79 24},
  pages={7704-8}
}
A recombinant plasmid containing a 1.2-kilobase cDNA for 3-hydroxy-3-methylglutaryl coenzyme A reductase was isolated from a cDNA library prepared from UT-1 cells, a clone of Chinese hamster ovary cells that has markedly elevated reductase activity. This plasmid, designated pRed-10, was identified by differential colony hybridization and hybrid-selected mRNA translation. The mRNA that hybridized to pRed-10 directed the synthesis in vitro of a 90,000-dalton protein that was immunoprecipitated by… CONTINUE READING

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