Molecular cloning into Tn5 and integration in the Pseudomonas aeruginosa chromosome: a tool for heterologous gene expression.

@article{Krishnapillai1986MolecularCI,
  title={Molecular cloning into Tn5 and integration in the Pseudomonas aeruginosa chromosome: a tool for heterologous gene expression.},
  author={Viji Krishnapillai and Alfred P{\"u}hler and Erich Lanka},
  journal={Journal of general microbiology},
  year={1986},
  volume={132 3},
  pages={707-15}
}
The DNA primase gene of the promiscuous IncP-1 conjugative plasmid RP1, encoding two polypeptides of 118 and 80 kDa, was inserted into the transposon Tn5 in Escherichia coli. The derivative transposon, Tn2523, was then transposed to a temperature-sensitive replication mutant of the promiscuous IncP-1 conjugative plasmid R68 at permissive temperature and the… CONTINUE READING