Molecular cloning and sequencing of the glycogen phosphorylase gene from Escherichia coli.

@article{Choi1989MolecularCA,
  title={Molecular cloning and sequencing of the glycogen phosphorylase gene from Escherichia coli.},
  author={Yoon La Choi and Makoto Kawamukai and Ryutaro Utsumi and Hiroshi Sakai and Teruya Komano},
  journal={FEBS letters},
  year={1989},
  volume={243 2},
  pages={193-8}
}
The glgP gene, which codes for glycogen phosphorylase, was cloned from a genomic library of Escherichia coli. The nucleotide sequence of the glgP gene contained a single open reading frame encoding a protein consisting of 790 amino acid residues. The glgP gene product, a polypeptide of Mr 87,000, was confirmed by SDS-polyacrylamide gel electrophoresis. The deduced amino acid sequence showed that homology between glgP of E. coli and rabbit glgP, human glgP, potato glgP, and E. coli malP was 48.6… CONTINUE READING