Molecular cloning and sequence of theBacillus stearothermophilus translational initiation factor IF2 gene

@article{Brombach1986MolecularCA,
  title={Molecular cloning and sequence of theBacillus stearothermophilus translational initiation factor IF2 gene},
  author={Martin Brombach and Claudio O. Gualerzi and Yoshikazu Nakamura and Cynthia L. Pon},
  journal={Molecular and General Genetics MGG},
  year={1986},
  volume={205},
  pages={97-102}
}
The structural gene for theBacillus stearothermophilus initiation factor IF2 was localized to a 6 kbHindIII restriction fragment by cross-hybridization with theSstI-SmaI fragment of theEscherichia coli infB gene. This fragment corresponds to the central region of the molecule containing the GTP-binding domain which is homologous inE. coli IF2, EF-Tu, EF-G and the humanras1 oncogene protein. After cloning into pACYC177, theHindIII fragment was further analysed by restriction mapping and cross… CONTINUE READING
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