Molecular cloning and sequence analysis of cDNA and genomic DNA for the human cone transducin alpha subunit.

@article{Kubo1991MolecularCA,
  title={Molecular cloning and sequence analysis of cDNA and genomic DNA for the human cone transducin alpha subunit.},
  author={Mitsumasa Kubo and Toshio Hirano and Mitsuaki Kakinuma},
  journal={FEBS letters},
  year={1991},
  volume={291 2},
  pages={245-8}
}
A novel GTP binding protein (G protein) alpha subunit cDNA was isolated from a T cell leukemia cell line, Jurkat, utilizing polymerase chain reaction (PCR). The predicted amino acid sequence of this G protein alpha subunit showed the highest identity (96.6%) to bovine cone cell-specific transducin (Tc alpha). The organization of the coding region of this G protein alpha subunit gene was composed of 8 exons and 7 introns. Northern hybridization revealed the presence of this G protein message in… CONTINUE READING