Molecular cloning and purification of an endochitinase from Serratia marcescens (Nima).

@article{RuizSanchez2005MolecularCA,
  title={Molecular cloning and purification of an endochitinase from Serratia marcescens (Nima).},
  author={Alejandro Ruiz-Sanchez and Ram{\'o}n Cruz-Camarillo and Rub{\'e}n Salcedo-Hern{\'a}ndez and Jorge E. Ibarra and Jos{\'e} E Barboza-Corona},
  journal={Molecular biotechnology},
  year={2005},
  volume={31 2},
  pages={103-11}
}
An endochitinase gene from the Serratia marcescens Nima strain (chiA Nima) was cloned, sequenced, and expressed in Escherichia coli DH5alphaF', and the recombinant protein (ChiA Nima) was purified by hydrophobic interaction chromatography. chiA Nima contains an open reading frame (ORF) that encodes an endochitinase with a deduced molecular weight and an isoelectric point of 61 kDa and 6.84, respectively. A sequence at the 5'-end was identified as a signal peptide, recognized by Gram-negative… CONTINUE READING