Molecular cloning and protein expression of EC1-2 and EC3-4 epitopes of pemphigus vulgaris antigen.

Abstract

OBJECTIVE To clone and express EC1-2 and EC3-4 epitopes of PVA (pemphigus vulgaris antigen, desmoglein-3) in order to diagnose pemphigus and study the relationship between epitopes of PVA and anti-PVA antibody. METHODS RNA was extracted from keratinocytes and the cDNA of epitopes EC1-2 and EC3-4 was synthesized by reverse transcription. Amplified genes of… (More)

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Cite this paper

@article{Zheng2000MolecularCA, title={Molecular cloning and protein expression of EC1-2 and EC3-4 epitopes of pemphigus vulgaris antigen.}, author={J Zheng and X Q Kang and W. D. Li and Y. Cheng and Moon-shong Tang and Wan Zhou}, journal={Chinese medical journal}, year={2000}, volume={113 11}, pages={1011-4} }