Molecular cloning and physical mapping of murine cytomegalovirus DNA.

@article{Ebeling1983MolecularCA,
  title={Molecular cloning and physical mapping of murine cytomegalovirus DNA.},
  author={August Ebeling and G{\"u}nther M. Keil and Elisabeth Knust and Ulrich Koszinowski},
  journal={Journal of virology},
  year={1983},
  volume={47 3},
  pages={
          421-33
        }
}
Murine cytomegalovirus (MCMV) Smith strain DNA is cleaved by restriction endonuclease HindIII into 16 fragments, ranging in size from 0.64 to 22.25 megadaltons. Of the 16 HindIII fragments, 15 were cloned in plasmid pACYC177 in Escherichia coli HB101 (recA). The recombinant plasmid clones were characterized by cleavage with the enzymes XbaI and EcoRI. In addition, fragments generated by double digestion of cloned fragments with HindIII and XbaI were inserted into the plasmid vector pACYC184… CONTINUE READING

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