Molecular cloning and nucleotide sequence of the gramicidin S synthetase 1 gene.

@article{Hori1989MolecularCA,
  title={Molecular cloning and nucleotide sequence of the gramicidin S synthetase 1 gene.},
  author={K. Hori and Y. Yamamoto and T. Minetoki and T. Kurotsu and M. Kanda and S. Miura and K. Okamura and J. Furuyama and Y. Saito},
  journal={Journal of biochemistry},
  year={1989},
  volume={106 4},
  pages={
          639-45
        }
}
  • K. Hori, Y. Yamamoto, +6 authors Y. Saito
  • Published 1989
  • Biology, Medicine
  • Journal of biochemistry
  • The entire gene for gramicidin S synthetase 1 (GS 1) was cloned into the plasmid vector pUC18, and the nucleotide sequences of the GS 1 gene and its flanking region were determined. The full-length clone was 4,539 base pairs long and had an open reading frame of 3,294 nucleotides coding for 1,098 amino acids. The calculated molecular weight of 123,474 agreed with the apparent molecular weight of 120,000 found in SDS-PAGE of GS 1 from B. brevis. The nucleotide sequence of GS 1 gene was highly… CONTINUE READING
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