Molecular cloning and nucleotide sequence of the alpha-toxin (phospholipase C) of Clostridium perfringens.

@article{Titball1989MolecularCA,
  title={Molecular cloning and nucleotide sequence of the alpha-toxin (phospholipase C) of Clostridium perfringens.},
  author={Richard W. Titball and S V Hunter and Kimberly L Martin and Bruce C Morris and A D Shuttleworth and T Rubidge and Don W Anderson and Daniel C. Kelly},
  journal={Infection and immunity},
  year={1989},
  volume={57 2},
  pages={367-76}
}
A fragment of DNA containing the gene coding for the phospholipase C (alpha-toxin) of Clostridium perfringens was cloned into Escherichia coli. The cloned DNA appeared to code only for the alpha-toxin and contained both the coding region and its associated gene promoter. The nucleotide sequence of the cloned DNA was determined, and an open reading frame was identified which encoded a protein with a molecular weight of 42,528. By comparison of the gene sequence with the N-terminal amino acid… CONTINUE READING
38 Extracted Citations
26 Extracted References
Similar Papers

Citing Papers

Publications influenced by this paper.
Showing 1-10 of 38 extracted citations

Referenced Papers

Publications referenced by this paper.
Showing 1-10 of 26 references

Cloning and sequencing of the gene encoding the phosphatidylcholine-preferring phospholipase C of Bacillus cereus

  • T. Johansen, T. Holme, P. H. Guddal, K. Sletten, F. B. Haugli, C. Little
  • Gene 65:293-304
  • 1988
Highly Influential
4 Excerpts

Toxins of Clostridium perfringens types

  • J. L. McDonel
  • 1986
Highly Influential
8 Excerpts

Synthesis of plasmid encoded polypeptides in maxicells

  • G. J. Boulnois, K. N. Timmis
  • 1984
1 Excerpt

Similar Papers

Loading similar papers…