Molecular cloning and nucleotide sequence of the alpha-toxin (phospholipase C) of Clostridium perfringens.

  title={Molecular cloning and nucleotide sequence of the alpha-toxin (phospholipase C) of Clostridium perfringens.},
  author={Richard W. Titball and S V Hunter and Kimberly L Martin and Bruce C Morris and A D Shuttleworth and T Rubidge and Don W Anderson and Daniel C. Kelly},
  journal={Infection and immunity},
  volume={57 2},
A fragment of DNA containing the gene coding for the phospholipase C (alpha-toxin) of Clostridium perfringens was cloned into Escherichia coli. The cloned DNA appeared to code only for the alpha-toxin and contained both the coding region and its associated gene promoter. The nucleotide sequence of the cloned DNA was determined, and an open reading frame was identified which encoded a protein with a molecular weight of 42,528. By comparison of the gene sequence with the N-terminal amino acid… CONTINUE READING
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