• Corpus ID: 35613997

Molecular cloning and nucleotide sequence analysis of encoded anti-insect toxin BotIT2 from the scorpion Buthus occitanus tunetanus venom.

@article{BelHajRhouma2003MolecularCA,
  title={Molecular cloning and nucleotide sequence analysis of encoded anti-insect toxin BotIT2 from the scorpion Buthus occitanus tunetanus venom.},
  author={Rahima Bel Haj Rhouma and Hafida Dkhil and Khadija Benkhadir and Lamia Borchani and Mohamed El Ayeb and Habib Karoui},
  journal={Archives de l'Institut Pasteur de Tunis},
  year={2003},
  volume={80 1-4},
  pages={
          35-41
        }
}
Numerous toxins from scorpion venoms are much more toxic to insects than to other animal classes, and possess high affinity to Na+ channels. Many of them active on insects were purified from the venom of Buthus occitanus tunetanus. Using amino acid sequences of BotIT2 and RACE-PCR amplification (Rapid amplification of cDNA ends) technique, we isolated, identified and sequenced the nucleotide sequence from the venom glands of the scorpion Buthus occitanus tunetanus. The cDNA encodes a precursor… 
Scorpion and spider venom peptides: gene cloning and peptide expression.