Molecular cloning and immunolocalization of a diuretic hormone receptor in rice brown planthopper (Nilaparvata lugens).

@article{Price2004MolecularCA,
  title={Molecular cloning and immunolocalization of a diuretic hormone receptor in rice brown planthopper (Nilaparvata lugens).},
  author={Daniel R. G. Price and Jingjing Du and Andrew Dinsmore and John A Gatehouse},
  journal={Insect molecular biology},
  year={2004},
  volume={13 5},
  pages={
          469-80
        }
}
RNA extracted from guts of rice brown planthopper, Nilaparvata lugens, was used to clone cDNA predicted to encode a diuretic hormone receptor (DHR). The DHR, a member of the calcitonin/secretin/corticotropin-releasing factor family of G-protein-coupled receptors, contains seven transmembrane domains and a large N-terminal extracellular domain potentially involved in hormone binding. The N-terminal domain was expressed as a recombinant protein, purified and used to raise antibodies. Anti-DHR IgG… CONTINUE READING

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