Molecular cloning and heterologous expression of an alpha-adrenergic-like octopamine receptor from the silkworm Bombyx mori.

@article{Ohtani2006MolecularCA,
  title={Molecular cloning and heterologous expression of an alpha-adrenergic-like octopamine receptor from the silkworm Bombyx mori.},
  author={Akifumi Ohtani and Yuichiro Arai and Fumiyo Ozoe and Hiroto Ohta and Kenji Narusuye and Jia Huang and Koh-ichi Enomoto and Hiroshi Kataoka and Akihiko Hirota and Yoshihisa Ozoe},
  journal={Insect molecular biology},
  year={2006},
  volume={15 6},
  pages={763-72}
}
A cDNA encoding an octopamine (OA) receptor (BmOAR1) was isolated from the nerve tissue of silkworm (Bombyx mori) larvae. Comparison of amino acid sequences showed that BmOAR1 is highly identical to OA receptors isolated from Periplaneta americana (Pa oa(1)), Apis mellifera (AmOA1), and Drosophila melanogaster (OAMB or DmOA1A). BmOAR1 was stably expressed in HEK-293 cells. OA above 1 microM led to an increase in intracellular cyclic AMP concentration ([cAMP](i)). The synthetic OA-receptor… CONTINUE READING
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