Molecular cloning and genetic analysis of a chloramphenicol acetyltransferase determinant from Clostridium difficile.

@article{Wren1988MolecularCA,
  title={Molecular cloning and genetic analysis of a chloramphenicol acetyltransferase determinant from Clostridium difficile.},
  author={Brendan W. Wren and Peter Mullany and Chris L. Clayton and Soad Tabaqchali},
  journal={Antimicrobial agents and chemotherapy},
  year={1988},
  volume={32 8},
  pages={1213-7}
}
A gene bank from a clinical isolate of Clostridium difficile expressing high chloramphenicol acetyltransferase activity was constructed by cloning Sau3A-cleaved clostridial DNA fragments into the plasmid vector pUC13. Among 1,020 clones tested, 11 were resistant to chloramphenicol; 1 of these, with an insert size of 1.9 kilobases (pPPM9), was studied further. The clone pPPM9 was mapped using a variety of restriction enzymes, and a 0.27-kilobase EcoRV-TaqI restriction fragment was shown to be… CONTINUE READING