Molecular cloning and functional expression of 5-HT1E-like rat and human 5-hydroxytryptamine receptor genes.

@article{Lovenberg1993MolecularCA,
  title={Molecular cloning and functional expression of 5-HT1E-like rat and human 5-hydroxytryptamine receptor genes.},
  author={T. Lovenberg and M. Erlander and B. Baron and M. Racke and A. L. Slone and B. W. Siegel and C. Craft and J. Burns and P. Danielson and J. Sutcliffe},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1993},
  volume={90},
  pages={2184 - 2188}
}
  • T. Lovenberg, M. Erlander, +7 authors J. Sutcliffe
  • Published 1993
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • Sequential polymerase chain reaction experiments were performed to amplify a unique sequence representing a guanine nucleotide-binding protein (G-protein)-coupled receptor from rat hypothalamic cDNA. Degenerate oligonucleotides corresponding to conserved amino acids from transmembrane domains III, V, and VI of known receptors [5-HT1A, 5-HT1C, and 5-HT2; 5-HT is serotonin (5-hydroxytryptamine)] were used as primers for the sequential reactions. The resulting product was subcloned and used to… CONTINUE READING
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