Molecular cloning and functional expression of cDNAs for Glycyrrhiza glabra squalene synthase.

@article{Hayashi1996MolecularCA,
  title={Molecular cloning and functional expression of cDNAs for Glycyrrhiza glabra squalene synthase.},
  author={Hideya Hayashi and Nobutsugu Hiraoka and Yasumasa Ikeshiro},
  journal={Biological & pharmaceutical bulletin},
  year={1996},
  volume={19 10},
  pages={1387-9}
}
We have isolated two cDNAs (GgSQS1 and GgSQS2) encoding squalene synthase of Glycyrrhiza glabra L. by cross-hybridization with that of Arabidopsis thaliana squalene synthase under conditions of low stringency. Their nucleotide sequences contained an open reading frame for a polypeptide of 413 amino acids (GgSQS1) and 412 amino acids (GgSQS2), respectively. The deduced amino acid sequence of GgSQS1 exhibits 88%, 81%, 78%, 45-44%, and 45-41% identity to those of the GgSQS2, Nicotiana benthamiana… CONTINUE READING