Molecular cloning and functional characterization of Argonaute-3 gene from Penaeus monodon.

  title={Molecular cloning and functional characterization of Argonaute-3 gene from Penaeus monodon.},
  author={Amnat Phetrungnapha and Teerapong Ho and Apinunt Udomkit and S. Panyim and C. Ongvarrasopone},
  journal={Fish & shellfish immunology},
  volume={35 3},
  • Amnat Phetrungnapha, Teerapong Ho, +2 authors C. Ongvarrasopone
  • Published 2013
  • Biology, Medicine
  • Fish & shellfish immunology
  • Argonaute (Ago) proteins play a crucial role in the shrimp RNA interference pathway. In this study, we identified and characterized a novel Ago gene from black tiger shrimp, Penaeus monodon. The complete open reading frame of P. monodon Ago3 (PmAgo3) consisted of 2559 nucleotides encoding a polypeptide of 852 amino acids with a predicted molecular weight of 97 kDa and an isoelectric point of 9.42. Analysis of the deduced amino acid sequence of PmAgo3 revealed the presence of two signature… CONTINUE READING
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