Molecular cloning and expression pattern of 14 kDa apolipoprotein in orange-spotted grouper, Epinephelus coioides.

@article{Zhou2005MolecularCA,
  title={Molecular cloning and expression pattern of 14 kDa apolipoprotein in orange-spotted grouper, Epinephelus coioides.},
  author={Li Zhou and Yang Wang and Bo Yao and Chuang-ju Li and Guan-Dong Ji and Jian-Fang Gui},
  journal={Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology},
  year={2005},
  volume={142 4},
  pages={432-7}
}
A novel fish-specific apolipoprotein (apo-14 kDa) has been recently cloned from eel and pufferfish. However, its expression pattern has not been elucidated. In this study, EcApo-14 has been screened from hypothalamic cDNA library of male orange-spotted grouper, which shows 62.9%, 51%, 46.9%, 43.2%, and 31.9% identities to Apo-14 of European flounder, pufferfish, Japanese eel, gibel carp, and grass carp, respectively. RT-PCR analysis reveals that this gene is first transcribed in neurula embryos… CONTINUE READING

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