Molecular cloning and expression of the fas ligand, a novel member of the tumor necrosis factor family

  title={Molecular cloning and expression of the fas ligand, a novel member of the tumor necrosis factor family},
  author={Takashi Suda and Tomohiro Takahashi and Pierre Golstein and Shigekazu Nagata},

Purification and characterization of the Fas-ligand that induces apoptosis

Results indicated that the 40-kD membrane glycoprotein expressed on PC60-d10S cells is the Fas-ligand that induces the apoptotic signal by binding to Fas.

CD27, a member of the tumor necrosis factor receptor family, induces apoptosis and binds to Siva, a proapoptotic protein.

It is shown that CD27, a member of the TNFR family, expressed on discrete subpopulations of T and B cells and known to provide costimulatory signals for T andB cell proliferation and B cell Ig production, can also induce apoptosis and co-crosslinking of surface Ig receptors along with ligation of CD27 augments CD27-mediated apoptosis.

Cloning and expression of a short Fas ligand: A new alternatively spliced product of the mouse Fas ligand gene.

RNase protection and immunoprecipitation analysis showed that FasLs is expressed in nonactivated normal spleen cells and in hybridoma T cells and that it is upregulated upon activation by anti-CD3 monoclonal antibody (MoAb).

Activated B cells express functional Fas ligand

Evidence that B lymphocytes can express FasL is provided and the data suggest that the Fas system may contribute to the control of B cell homeostasis.

Tumor Necrosis Factor-α and Interferon-γ Induce Expression of Functional Fas Ligand on HT29 and MCF7 Adenocarcinoma Cells

It is demonstrated that TNF-α and IFN-γ induce expression of functional FasL in adenocarcinoma cells which thereby can kill T lymphocytes by the FasL/Fas pathway.

Swapping between Fas and Granulocyte Colony-stimulating Factor Receptor*

The results indicated that receptors belonging to different receptor families can be functionally exchanged and confirm that a homodimer of G-CSFR can transduce the growth signal, whereas Fas must be oligomerized (probably trimerized) to transduces the apoptotic signal.

Apoptosis mediated by the Fas system.

  • S. Nagata
  • Biology, Medicine
    Progress in molecular and subcellular biology
  • 1996
Findings suggest that the Fas system plays a role not only in the physiological process of lymphocyte development, but also in the cytotoxic T-lymphocyte-mediated disease such as fulminant hepatitis.

Cloning and characterization of the bovine Fas.

To investigate the apoptotic activity of bFAs, MDBK and bFas-transfected L929 cells were exposed to a monoclonal anti-hFas IgM, and like other cell culture systems, the antibody failed to trigger cell death in MDBk andbFas transfecting L 929 cells.

Different apoptotic pathways mediated by Fas and the tumor-necrosis-factor receptor. Cytosolic phospholipase A2 is not involved in Fas-mediated apoptosis.

It is indicated that cPLA2 is required for TNF-induced apoptosis, whereas it is dispensable for Fas-mediated apoptosis and suggested that the TNF receptor and Fas use different signaling pathways for apoptosis.



Effect of bcl-2 on Fas antigen-mediated cell death.

The results suggest that the Fas Ag and TNF receptor may share the same signaling pathway, and that bcl-2 interferes with the apoptotic process mediated by the FasAg and T NF receptor.

Molecular and biological characterization of a murine ligand for CD40

The cloning of a ligand for CD40 that is expressed on the cell surface of activated T cells and mediates B-cell proliferation in the absence of co-stimulus, as well as IgE production in the presence of interIeukin-4 is reported.

The cDNA structure, expression, and chromosomal assignment of the mouse Fas antigen.

Interspecific backcross analysis indicated that the gene coding for the Fas antigen is in the distal region of mouse chromosome 19, and was significantly induced by treatment with IFN-gamma but not byIFN-alpha/beta.

Lethal effect of the anti-Fas antibody in mice

The findings suggest that the Fas antigen is important in programmed cell death in the liver, and may be involved in fulminant hepatitis in some cases.

A cell-killing monoclonal antibody (anti-Fas) to a cell surface antigen co-downregulated with the receptor of tumor necrosis factor

It is suggested that the cell-killing activity of TNF is mediated by Fas antigen associated with the TNF-R, an mAb specific for a human cell surface component (termed anti-Fas mAb).