Molecular cloning and expression of the CRISP family of proteins in the boar.

@article{Vadnais2008MolecularCA,
  title={Molecular cloning and expression of the CRISP family of proteins in the boar.},
  author={Melissa L Vadnais and Douglas N. Foster and Kenneth P. Roberts},
  journal={Biology of reproduction},
  year={2008},
  volume={79 6},
  pages={1129-34}
}
The family of mammalian cysteine-rich secretory proteins (CRISP) have been well characterized in the rat, mouse, and human. Here we report the molecular cloning and expression analysis of CRISP1, CRISP2, and CRISP3 in the boar. A partial sequence published in the National Center for Biotechnology Information (NCBI) database was used to derive the full-length sequences for CRISP1 and CRISP2 using rapid amplification of cDNA ends. RT-PCR confirmed the expression of these mRNAs in the boar… CONTINUE READING