Molecular cloning and expression of murine thromboxane synthase.

  title={Molecular cloning and expression of murine thromboxane synthase.},
  author={L Zhang and Michael B. Chase and R F Shen},
  journal={Biochemical and biophysical research communications},
  volume={194 2},
The complementary DNA (cDNA) for murine thromboxane synthase (TS) was isolated from a lung cDNA library. The full-length cDNA (1,910 bp) encodes a 533 amino acid protein (Mr 58,220) sharing 78% identity with human TS. Sequence comparison indicated that one of the two N-glycosylation sites, eight of the eleven cysteine residues, and a heme-binding domain are conserved in both murine and human TS sequences. The authenticity of the cDNA was confirmed by transient expression of a catalytically… Expand
The porcine thromboxane synthase-encoding cDNA: sequence, mRNA expression and enzyme production in Sf9 insect cells.
A high-titer recombinant baculovirus containing pTS cDNA was developed to conduct a time course study of enzyme production in Spodoptera frugiperda (Sf9) cells and produced TS resembles purified pTS in catalysis, as well as inhibition by a substrate analog inhibitor. Expand
Genomic organization, chromosomal localization, and expression of the murine thromboxane synthase gene.
These studies provide the genetic tools and information for TS research in mice, which should expedite understanding of the genetic contribution of TS in normal physiology as well as in disease states. Expand
Genomic structure and polymorphism of the human thromboxane synthase-encoding gene.
Southern analysis indicates that the human haploid genome contains a single copy of the TS gene, and transcription of hTS is primarily TATA-independent, as determined by promoter-directed reporter gene expression in transfected cells. Expand
Abundant expression of thromboxane synthase in rat macrophages
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Expression, Purification, and Spectroscopic Characterization of Human Thromboxane Synthase*
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Rat kidney thromboxane synthase: cDNA cloning and gene expression regulation in hydronephrotic kidney.
The structure of rat kidney TX synthase is revealed, which may cause stimulation of TX synthesis, and is possibly implicated in the tissue injury in hydronephrotic kidney. Expand
Transcriptional Control of the Human Thromboxane Synthase Genein Vivo and in Vitro *
Promoter analyses of human TXAS using in vitro transcription and in vivo transfection methods indicate that binding of NF-E2 is critical both for alteration of the nucleosomal structure and for activation of the TXAS promoter, whereas the TATA and initiate elements are essential for transcription. Expand
Identification of Thromboxane A2 Synthase Active Site Residues by Molecular Modeling-guided Site-directed Mutagenesis*
The present results from site-directed mutagenesis support the overall structure of the TXAS active site predicted by homology modeling and have allowed refinement of the position of bound substrate. Expand
In situ localization and regulation of thromboxane A(2) synthase in normal and LPS-primed lungs.
It is concluded that the pulmonary distribution of TxA(2) synthase displays close similarity between rat and human lung tissues and matches well with the previously described immunolocalization of cyclooxygenase-1 and cyclo oxygenase-2 in this tissue. Expand
Identification of genetic variants in the human thromboxane synthase gene (CYP5A1).
Eleven polymorphisms have been identified in the CYP5A1 gene including eight missense mutations R61H, D161E, N246S, L357V, Q417E, E450K, T451N and R466Q, the first report of genetic variants in the human CYP 5A1 altering the protein sequence. Expand