Molecular cloning and expression of cDNAs encoding human alpha-mannosidase II and a previously unrecognized alpha-mannosidase IIx isozyme.

@article{Misago1995MolecularCA,
  title={Molecular cloning and expression of cDNAs encoding human alpha-mannosidase II and a previously unrecognized alpha-mannosidase IIx isozyme.},
  author={M Misago and Y F Liao and Shinichi Kudo and Susumu Eto and M. G. Matt{\'e}i and Kelley W Moremen and Michiko N. Fukuda},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1995},
  volume={92 25},
  pages={11766-70}
}
Golgi alpha-mannosidase II (alpha-MII) is an enzyme involved in the processing of N-linked glycans. Using a previously isolated murine cDNA clone as a probe, we have isolated cDNA clones encompassing the human alpha-MII cDNA open reading frame and initiated isolation of human genomic clones. During the isolation of genomic clones, genes related to that encoding alpha-MII were isolated. One such gene was found to encode an isozyme, designated alpha-MIIx. A 5-kb cDNA clone encoding alpha-MIIx was… CONTINUE READING