Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor

@article{Nagata1986MolecularCA,
  title={Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor},
  author={S. Nagata and M. Tsuchiya and S. Asano and Y. Kaziro and T. Yamazaki and O. Yamamoto and Y. Hirata and N. Kubota and M. Oheda and H. Nomura and M. Ono},
  journal={Nature},
  year={1986},
  volume={319},
  pages={415-418}
}
Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of hormone-like glycoproteins that regulate haematopoietic cell proliferation and differentiation1,2, and G-CSF almost exclusively stimulates the colony formation of granulocytes from committed precursor cells in semi-solid agar culture2. Recently, Nomura et al.3 have established a human squamous carcinoma cell line (designated CHU-2) from a human oral cavity tumour which produces large quantities of CSF constitutively… Expand
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