Molecular cloning and expression of an IL-6 signal transducer, gp130

@article{Hibi1990MolecularCA,
  title={Molecular cloning and expression of an IL-6 signal transducer, gp130},
  author={Masahiko Hibi and Masaaki Murakami and Mikiyoshi Saito and Toshio Hirano and Tetsuya Taga and Tadamitsu Kishimoto},
  journal={Cell},
  year={1990},
  volume={63},
  pages={1149-1157}
}

Figures from this paper

Activation of the signal transducer glycoprotein 130 by both IL-6 and IL-11 requires two distinct binding epitopes.
TLDR
There is a substantial difference in the mechanism of receptor engagement by cytokines that signal via gp130, suggesting a common mode of recognition of helical cytokines by class I cytokine receptors.
Structure of an Extracellular gp130 Cytokine Receptor Signaling Complex
TLDR
The cross-reactivity of gp130 is apparently due to a chemical plasticity evident in the amphipathic gp130 cytokine-binding sites, which enhances the complementarity of the viral IL-6–gp130 binding interfaces.
IL-6 receptor and mechanism of signal transduction.
The N-terminus of gp130 is critical for the formation of the high-affinity interleukin-6 receptor complex.
TLDR
It is demonstrated, using biosensor analysis and size-exclusion chromatography, that modification of the N-terminus of sgp130 interferes with the in vitro in solution formation of the stable hexameric IL-6 receptor complex, and the critical involvement of theN-terminal Ig-like domain of gp130 in tethering the two trimers to form the stablehexamer.
Molecular cloning and expression of the human interleukin 5 receptor
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The human IL-5 receptor characterized in this paper is essential for signal transduction, because expression of this molecule in murine IL-3-dependent cell line FDC-P1 allowed these cells to proliferate in response to IL- 5.
Interleukin-6 Family of Cytokines Induced Activation of Different Functional Sites Expressed by gp130 Transducing Protein*
TLDR
It is indicated that in addition to functional site(s) required by the whole family of IL-6 type cytokines to transduce the signal insight the cell, specific cognate functional sites were recruited by OSM, CNTF, or IL-11.
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Interleukin-6 triggers the association of its receptor with a possible signal transducer, gp130
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