Molecular cloning and expression of a xylanase gene from Cellulomonas sp. into Escherichia coli

@article{Bhalerao2004MolecularCA,
  title={Molecular cloning and expression of a xylanase gene from Cellulomonas sp. into Escherichia coli},
  author={Jayant Bhalerao and Akash Patki and Mrinal R. Bhave and Ishant Khurana and Dileep N. Deobagkar},
  journal={Applied Microbiology and Biotechnology},
  year={2004},
  volume={34},
  pages={71-76}
}
The xylanase gene of Cellulomonas sp. NCIM 2353 was cloned in pUC 18 and selected by growth on xylan as the sole carbon source. The functional clone harboured the recombinant plasmid with an insert of 1.42 kbp, as determined by restriction mapping and Southern hydridization. The clone secreted a xylanase of 45 000 mol. wt. as determined by Western blot analysis using specific antixylanase antibodies. The DNA insert carried the full structural gene along with its promoter and possibly regulatory… CONTINUE READING