Molecular cloning and expression of a locus (mdoA) implicated in the biosynthesis of membrane-derived oligosaccharides in Escherichia coli.

@article{Lacroix1989MolecularCA,
  title={Molecular cloning and expression of a locus (mdoA) implicated in the biosynthesis of membrane-derived oligosaccharides in Escherichia coli.},
  author={J{\'e}r{\^o}me Lacroix and Marc Temp{\^e}te and Bernadette Menichi and J. P. Bohin},
  journal={Molecular microbiology},
  year={1989},
  volume={3 9},
  pages={1173-82}
}
Mutants of Escherichia coli defective in the mdoA locus are blocked at an early stage in the biosynthesis of membrane-derived oligosaccharides. The mdoA locus has now been cloned into multicopy plasmids. A 5 kb DNA fragment is necessary to complement mdoA mutations. Cells harbouring the mdoA+ plasmid produced three to four times more MDO than wild-type cells. MDO overproduction did not affect the degree of MDO substitution with sn-1-phosphoglycerol residues. The biosynthesis of MDO remained… CONTINUE READING

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