Molecular cloning and expression of a cDNA encoding the luciferase from the firefly, Hotaria unmunsana.

@article{Choi2002MolecularCA,
  title={Molecular cloning and expression of a cDNA encoding the luciferase from the firefly, Hotaria unmunsana.},
  author={Y. S. Choi and K. Lee and J. S. Bae and S. R. Kim and I. Kim and S. M. Lee and H. Sohn and B. Jin},
  journal={Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology},
  year={2002},
  volume={132 3},
  pages={
          661-70
        }
}
  • Y. S. Choi, K. Lee, +6 authors B. Jin
  • Published 2002
  • Biology, Medicine
  • Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
We have cloned and characterized a cDNA encoding the luciferase from the firefly, Hotaria unmunsana. The cDNA encoding the luciferase of H. unmunsana was isolated by RT-PCR with a gene-specific primer set. The cDNA encoding the luciferase of H. unmunsana is 1644 base pairs long with an open reading frame of 548 amino acid residues. The deduced amino acid sequence of the luciferase gene of H. unmunsana showed 98.0 and 96.8% identity to H. parvula and Luciola mingrelica, respectively… Expand
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