Molecular cloning and expression of Chlamydia trachomatis major outer membrane protein antigens in Escherichia coli.

@article{Stephens1985MolecularCA,
  title={Molecular cloning and expression of Chlamydia trachomatis major outer membrane protein antigens in Escherichia coli.},
  author={Richard S. Stephens and Cho-chou Kuo and George Newport and Nina Agabian},
  journal={Infection and immunity},
  year={1985},
  volume={47 3},
  pages={713-8}
}
DNA obtained from Chlamydia trachomatis (serovar L2) was partially digested with DNase I and inserted into the beta-galactosidase gene of bacteriophage lambda gt11. Seven recombinants were selected that produced immunoreactive fusion proteins which were detected with anti-C. trachomatis rabbit serum. One recombinant, designated lambda gt11/L2/33, reacted… CONTINUE READING