Molecular cloning and developmental expression of foxP2 in zebrafish

@article{Bonkowsky2005MolecularCA,
  title={Molecular cloning and developmental expression of foxP2 in zebrafish},
  author={Joshua L. Bonkowsky and Chi-Bin Chien},
  journal={Developmental Dynamics},
  year={2005},
  volume={234}
}
Forkhead domain transcription factors are a large gene family with multiple roles in development. FOXP2, a recently identified member of this family, has been shown to be critical for normal development of language in humans, but little is known of its broader function during nervous system development. We report here the cloning of foxP2, the zebrafish ortholog of FOXP2. Zebrafish FoxP2 is highly conserved in its zinc‐finger and forkhead domains, but lacks the large glutamine repeat… 

Molecular cloning, characterization, and developmental expression of foxp1 in zebrafish

Results of whole-mount in situ hybridization showed that foxp1 exhibits very complex and dynamic expression pattern during early embryonic development, providing evidence thatfoxp1 is likely to function as a very important transcription factor in the development of the central nervous system and many other organs in zebrafish.

Expression of FoxP2 during zebrafish development and in the adult brain.

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Zebrafish foxP2 Zinc Finger Nuclease Mutant Has Normal Axon Pathfinding

It is suggested that foxP2 is not necessary for axon pathfinding during development, and when examined using a pan-axonal marker or transgenic lines, including afoxP2-neuron-specific enhancer, it did not observe any axon guidance errors.

The medaka FoxP2, a homologue of human language gene FOXP2, has a diverged structure and function.

Results suggest that medaka FoxP2 may play a different function in the development of the medaka fish, after it was found that the three amino acids of forkhead domain were responsible for the weak repressive activity.

Transcriptional Enhancers in the FOXP2 Locus Underwent Accelerated Evolution in the Human Lineage.

The results indicate that regulatory sequences in the FOXP2 locus underwent a human-specific evolutionary process suggesting that the transcriptional machinery controlling this gene could have also evolved differentially in the human lineage.

Domain-specific regulation of foxP2 CNS expression by lef1

This work shows that lef1 is necessary for expression of foxP2 in the tectum, mid-hindbrain boundary, and hindbrain during CNS development, and is the first insight into the upstream regulation offoxP2 during development.

Characterization of Foxp2 functions in the mouse cortex

The results deliver first insights into cortical Foxp2 dependent functions in mouse social behaviors and provides a rational basis for further mechanistic studies of the ancestral functions of cortical FoxP2 that may have been recruited during speech and language evolution.

Generation of mice with a conditional Foxp2 null allele

It is demonstrated that early global Cre‐mediated recombination yields a null allele, as shown by loss of the loxP‐flanked exons at the RNA level and an absence of Foxp2 protein.

Characterization of transcription factors in monogenic disorders of speech and language

It is demonstrated thatFOXP2 is SUMOylated at a single major site, which is conserved in all FOXP2 vertebrate orthologues and in the paralogues FOXP1 and FOXP4, and which suggests a potential role forsumOylation in regulating FoxP2 activity in vivo.
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References

SHOWING 1-10 OF 34 REFERENCES

Expression of Foxp2, a gene involved in speech and language, in the developing and adult striatum

The striatum is known to be involved in the process of procedural memory, and mutation of Foxp2 results in neurological disorders of language and speech, so the striatum, particularly the striosomal system, may participate in neural information processing for language and Speech.

Characterization of Foxp2 and Foxp1 mRNA and protein in the developing and mature brain

The time course and localization of Foxp2 and Foxp1 mRNA and protein expression in the developing and adult mouse using in situ hybridization and immunohistochemistry are investigated and a role for these genes in postmigratory neuronal differentiation is suggested.

Two divergent slit1 genes in zebrafish

The cloning of full‐length cDNAs for two zebrafish slit orthologs, slit1a and slit1b, are described, suggesting that Slit proteins may have roles in tissue morphogenesis in addition to their established roles in axon guidance and cell migration.

Characterization of a New Subfamily of Winged-helix/Forkhead (Fox) Genes That Are Expressed in the Lung and Act as Transcriptional Repressors*

Two members of the Fox gene family are identified and characterized, Foxp1 and Foxp2, and it is shown that they comprise a new subfamily of Fox genes expressed in the lung and are important regulators of lung epithelial gene transcription.

Cloning and expression of three zebrafish roundabout homologs suggest roles in axon guidance and cell migration

Analysis of extracellular domains and conserved cytoplasmic motifs shows that zebrafish Robo1 and Robo2 are orthologs of mammalian Robo1and Robo2, respectively, while zebra fish Robo3 is likely to be an ortholog of mouse Rig‐1.

FOXP2 expression during brain development coincides with adult sites of pathology in a severe speech and language disorder.

The homologous pattern of FOXP2/Foxp2 expression in human and mouse argues for a role for this gene in development of motor-related circuits throughout mammalian species.

Molecular evolution of FOXP2, a gene involved in speech and language

It is shown that human FOXP2 contains changes in amino-acid coding and a pattern of nucleotide polymorphism, which strongly suggest that this gene has been the target of selection during recent human evolution.

Structural analysis of disease‐causing mutations in the P‐subfamily of forkhead transcription factors

The missense mutations R553H inFOXP2 and R397W in FOXP3 dramatically alter the electrostatic potentials of the molecular surface of their respective forkhead domains, which have an impact on the three‐dimensional structure, stability, and surface electrostatic charge distribution of the Forkhead domains.

Accelerated protein evolution and origins of human-specific features: Foxp2 as an example.

An important role that FOXP2 may have played in the origin of human speech is suggested and a strategy for identifying candidate genes underlying the emergences of human-specific features is demonstrated.