Molecular cloning and chromosomal assignment of a human perforin (PFP) gene

  title={Molecular cloning and chromosomal assignment of a human perforin (PFP) gene},
  author={Yoichi Shinkai and Michihiro C. Yoshida and Keiko Maeda and Tetsuji Kobata and Kazuo Maruyama and Junji Yodoi and H. Yagita and Ko Okumura},
Human perforin cDNA was isolated and the complete nucleotide sequence of the gene determined. The deduced amino acid sequence of human perforin showed 68.4% similarity to that of mouse perforin. RNA blot analysis of the human perforin gene revealed that the gene product is expressed preferentially in killer-type cells among cell lines tested, and in large granular lymphocytes among the peripheral blood mononuclear cells. In situ hybridization analysis with a human perforin cDNA probe revealed… CONTINUE READING
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