Molecular cloning and characterization of two 1-deoxy-d-xylulose-5-phosphate synthase genes involved in tanshinone biosynthesis in Salvia miltiorrhiza

@article{Zhou2016MolecularCA,
  title={Molecular cloning and characterization of two 1-deoxy-d-xylulose-5-phosphate synthase genes involved in tanshinone biosynthesis in Salvia miltiorrhiza},
  author={Wei Zhou and Fenfen Huang and Shen Li and Yang Wang and Congcong Zhou and Min Shi and Jing Wang and Yingjie Chen and Yao Wang and Hui Wang and Guoyin Kai},
  journal={Molecular Breeding},
  year={2016},
  volume={36},
  pages={1-12}
}
The synthesis of diterpenoid tanshinones, which are some of the main bioactive ingredients of Danshen (the roots of Salvia miltiorrhiza), begins with the plastidial methylerythritol 4-phosphate (MEP) pathway. 1-Deoxy-d-xylulose-5-phosphate synthase (DXS) catalyzes the first step of the MEP pathway and is considered to be a key rate-limiting enzyme. To confirm the function of DXS genes, in this study two isoforms of DXS (SmDXS1 and SmDXS2) were isolated by rapid amplification of DNA ends from… CONTINUE READING