Molecular cloning and characterization of two voltage‐gated K+ channel cDNAs from human ventricle

@article{Tamkun1991MolecularCA,
  title={Molecular cloning and characterization of two voltage‐gated K+ channel cDNAs from human ventricle},
  author={Michael M. Tamkun and Karen M. Knoth and Julia A. Walbridge and Heyo K. Kroemer and Dan M. Roden and Deborah M. Glover},
  journal={The FASEB Journal},
  year={1991},
  volume={5},
  pages={331 - 337}
}
K+ channels represent the most complex class of voltage‐gated ion channels from both functional and structural standpoints. In the heart these channels are responsible for the rapid repolarizing phases of the action potential and are the targets of several antiarrhythmic drugs. Full‐length cDNA clones were isolated from human ventricular libraries that encode two voltage‐gated K+ channels. These two cDNAs, designated HK1 and HK2, encode proteins of 653 and 605 amino acids, respectively. HK1 is… 
Functional expression of an inactivating potassium channel cloned from human heart.
Recently a putative K+ channel with homology to the Shaker family of potassium channels has been cloned from human ventricular myocardium. However, proof that the cDNA encodes a K+ channel requires
Molecular Biology of the Voltage‐Gated Potassium Channels of the Cardiovascular System
TLDR
The application of molecular cloning technology to cardiovascular K+ channels has identified the primary structure of these proteins, and heterologous expression systems have allowed a detailed analysis of channel function and pharmacology without contaminating currents.
Cloning and characterization of a Kv1.5 delayed rectifier K+ channel from vascular and visceral smooth muscles.
TLDR
The expression of a Kv1.5 K+ channel from canine colonic smooth muscle demonstrated a channel highly selective for K+, which activates in a voltage-dependent manner on depolarization to membrane potentials positive to -40 mV and is proposed to represent a component of the delayed rectifier current in both vascular and visceral smooth muscles.
Heterogeneity in K+ Channel Transcript Expression Detected in Isolated Ferret Cardiac Myocytes
TLDR
The results show that both the diversity and heterogeneity of K+ channel mRNA in heart tissue is greater than previously suspected.
A rapidly activating and slowly inactivating potassium channel cloned from human heart. Functional analysis after stable mammalian cell culture expression
TLDR
The electrophysiological properties of HK2 (Kv1.5), a K+ channel cloned from human ventricle, were investigated after stable expression in a mouse Ltk- cell line, indicating expression of a single population of K+ channels.
Heteromultimeric assembly of human potassium channels. Molecular basis of a transient outward current?
TLDR
The results demonstrate that expression of different K+ channel genes can produce channel protein subunits that assemble as heteromultimers with unique properties, and it is shown that certain combinations of voltage-gated K+ channels probably do not contribute to native transient outward current.
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References

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TLDR
Functional characterization of the HBK 2/RCK2 K+ channels in Xenopus laevis oocytes following micro‐injection in in vitro transcribed HBK2 or RCK2 cRNA showed that theHBK2/RCk2 proteins form voltage‐gated K+annels with novel functional and pharmacological properties.
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TLDR
Cloning and sequencing of cDNAs isolated from a rat cortex cDNA library reveals that a gene family encodes several highly homologous K+ channel forming (RCK) proteins, which suggest the molecular basis for the diversity of voltage‐gated K+ channels in mammalian brain is based on the expression of several RCK proteins by a family of genes.
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TLDR
It is reported here that heteromultimeric K+ channels composed of two different RCK proteins (RCK1 and RCK4) assemble after cotransfection of HeLa cells with the corresponding cDNAs and after coinjection of the respective cRNAs into Xenopus oocytes.
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TLDR
The cloning of RKShIIIA is reported, a cDNA encoding a K+ channel sequence expressed in rat brain that cannot be assigned to either of the two known classes of Sh-family genes in mammals based on sequence analysis.
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TLDR
The isolation of complementary DNA clones from the mouse brain are reported, the nucleotide sequences of which predict a protein remarkably similar to the Shaker protein, suggesting that these mouse clones encode a potassium channel component and that the conserved amino acids may be essential to some aspect of potassium channel function.
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TLDR
In vitro transcripts made from a directional complementary DNA library are isolated, by expression cloning in Xenopus oocytes, a novel K+-channel gene (drk1), which encodes channels that are K+ selective and belong to the delayed rectifier class of channels, rather than the A-type class encoded by the Shaker gene of Drosophila.
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TLDR
Evidence that the Shaker A-type K+ channels expressed in Xenopus oocytes contain several Shaker polypeptides is provided, and it is suggested that heteromultimer formation may increase K+ channel diversity beyond even the level expected from the large number of K+Channel genes and alternative splicing products.
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