Molecular cloning and characterization of three cDNAs encoding putative mitogen-activated protein kinase kinases (MAPKKs) in Arabidopsis thaliana.

@article{Ichimura1998MolecularCA,
  title={Molecular cloning and characterization of three cDNAs encoding putative mitogen-activated protein kinase kinases (MAPKKs) in Arabidopsis thaliana.},
  author={Kazuya Ichimura and Tsuyoshi Mizoguchi and Nobuaki Hayashida and Motoaki Seki and Kazuo Shinozaki},
  journal={DNA research : an international journal for rapid publication of reports on genes and genomes},
  year={1998},
  volume={5 6},
  pages={341-8}
}
We isolated three Arabidopsis thaliana cDNA clones (ATMKK3, ATMKK4 and ATMKK5) encoding protein kinases with extensive homology to the mitogen-activated protein kinase kinases (MAPKKs) of various organisms in the catalytic domain. ATMKK3 shows high homology (85% identity) to NPK2, a tobacco MAPKK homologue. ATMKK4 and 5 are closely related to each other (84% identity). Phylogenetic analysis showed that the plant MAPKKs constitute at least three subgroups. The recombinant ATMKK3 and ATMKK4 were… CONTINUE READING
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