Molecular cloning and characterization of the beta-amylase gene from Bacillus circulans.

@article{Siggens1987MolecularCA,
  title={Molecular cloning and characterization of the beta-amylase gene from Bacillus circulans.},
  author={K W Siggens},
  journal={Molecular microbiology},
  year={1987},
  volume={1 1},
  pages={86-91}
}
A gene encoding the beta-amylase of Bacillus circulans was isolated from a lambda library and sequenced. The structural gene consists of a 1725 bp open reading frame encoding a polypeptide with a predicted molecular wt of 62830 Daltons. Two active forms of the enzyme were found when the gene was expressed in E. coli. The larger 60 kD form was approximately 3 kD larger than the mature beta-amylase secreted from B. circulans, suggesting that processing of this protein is different between the two… CONTINUE READING

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