Molecular cloning and characterization of the promoter for human type-1 angiotensin II receptor gene.

@article{Takayanagi1994MolecularCA,
  title={Molecular cloning and characterization of the promoter for human type-1 angiotensin II receptor gene.},
  author={Ryoichi Takayanagi and Keizo Ohnaka and Yasuyoshi Sakai and Shoichiro Ikuyama and Hajime Nawata},
  journal={Biochemical and biophysical research communications},
  year={1994},
  volume={200 3},
  pages={1264-70}
}
We isolated a genomic clone containing 2558 bp of the 5'-flanking region and 217 bp of the first exon for the human type-1 angiotensin II receptor (AT1) gene. Primer extension and RNAase protection analyses identified a transcriptional start site (+1) at 39 and 114 bp downstream of putative TATA and GC boxes, respectively. Chimeras containing 2.6 kbp(-2558 to +79) of the 5'-flanking region and chloramphenicol acetyltransferase (CAT) gene expressed a significant CAT activity when transfected… CONTINUE READING

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