Molecular cloning and characterization of six genes, determination of gene order and intergenic sequences and leader sequence of mumps virus.

@article{Elango1988MolecularCA,
  title={Molecular cloning and characterization of six genes, determination of gene order and intergenic sequences and leader sequence of mumps virus.},
  author={Narayanasamy Elango and Tamas M. Varsanyi and Jan K{\"o}vamees and E. C. J. Norrby},
  journal={The Journal of general virology},
  year={1988},
  volume={69 ( Pt 11)},
  pages={
          2893-900
        }
}
mRNA isolated from mumps virus-infected Vero cells was converted into cDNA and cloned into the PstI site of the plasmid pBR322. After screening with 32P-labelled cDNA synthesized from poly(A)+ RNA of uninfected or mumps virus-infected Vero cells, five different groups of virus-specific clones were obtained. The virus specificity of the clones was confirmed by Northern blot analysis, in which the cDNA inserts from the five different groups hybridized to mRNAs of about 2100, 1500, 1450, 2000 and… CONTINUE READING

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