Molecular cloning and characterization of rpbA encoding RNA polymerase II largest subunit from a filamentous fungus, Aspergillus oryzae.

@article{Nakajima2000MolecularCA,
  title={Molecular cloning and characterization of rpbA encoding RNA polymerase II largest subunit from a filamentous fungus, Aspergillus oryzae.},
  author={Keiji Nakajima and Yen-Pei Christy Chang and Tohru S. Suzuki and Y. Jigami and Masayuki Machida},
  journal={Bioscience, biotechnology, and biochemistry},
  year={2000},
  volume={64 3},
  pages={641-6}
}
We have cloned rpbA encoding the RNA polymerase II largest subunit (polIIL) from a filamentous fungus, Aspergillus oryzae. The rpbA product included eight highly conserved regions and the carboxyl-terminal domain (CTD). A. oryzae polIIL CTD with 184 amino acids was composed of 25 CTD consensus repeats, which was a similar number to those of lower eukaryotes. The amino acids in each repeat of A. oryzae polIIL, however, conformed less to the CTD consensus than those of polIILs from other lower… CONTINUE READING