Molecular cloning and characterization of prolyl endopeptidase from human T cells.

@article{Shirasawa1994MolecularCA,
  title={Molecular cloning and characterization of prolyl endopeptidase from human T cells.},
  author={Yumi Shirasawa and Toshiaki Osawa and A. Hirashima},
  journal={Journal of biochemistry},
  year={1994},
  volume={115 4},
  pages={724-9}
}
The prolyl endopeptidase (PEP) gene of human T cells was amplified by the PCR method and cloned in Escherichia coli. The complete gene consisted of 2,130 nucleotides corresponding to 710 amino acid residues with a calculated molecular mass of 80,750. The nucleotide sequence of this clone revealed that T cell PEP DNA is 48, 50, and 91% homologous to those of Flavobacterium meningosepticum, Aeromonas hydrophila, and porcine brain PEP, respectively. This gene was fused to the lacZ sequence from E… CONTINUE READING

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The complete gene consisted of 2,130 nucleotides corresponding to 710 amino acid residues with a calculated molecular mass of 80,750 .
The complete gene consisted of 2,130 nucleotides corresponding to 710 amino acid residues with a calculated molecular mass of 80,750 .
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