Molecular cloning and characterization of lactate dehydrogenase gene from Eimeria tenella

Abstract

Lactate dehydrogenase (LDH) is a key enzyme in the glycolytic pathway and is crucial for parasite survival. In this study, we cloned and expressed the LDH of Eimeria tenella (EtLDH). Real-time polymerase chain reaction and Western blot analysis revealed that the expression of EtLDH was developmentally regulated at the messenger RNA (mRNA) and protein levels… (More)
DOI: 10.1007/s00436-014-3953-2

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