Molecular cloning and characterization of genes required for ribose transport and utilization in Escherichia coli K-12

@article{Iida1984MolecularCA,
  title={Molecular cloning and characterization of genes required for ribose transport and utilization in Escherichia coli K-12},
  author={A Iida and Shigeaki Harayama and Tetsuo Iino and Gerald L. Hazelbauer},
  journal={Journal of Bacteriology},
  year={1984},
  volume={158},
  pages={674 - 682}
}
We isolated spontaneous and transposon insertion mutants of Escherichia coli K-12 that were specifically defective in utilization or in high-affinity transport of D-ribose (or in both). Cotransduction studies located all of the mutations near ilv, at the same position as previously identified mutations causing defects in ribokinase ( rbsK ) or ribose transport ( rbsP ). Plasmids that complemented the rbs mutations were isolated from the collection of ColE1 hybrid plasmids constructed by Clarke… 
D-ribose metabolism in Escherichia coli K-12: genetics, regulation, and transport.
TLDR
The high-affinity transport-defective mutants were able to utilize D-ribose, indicating that at least a second, low-Affinity transport system for D- ribose is present in Escherichia coli K-12.
A Mutated PtsG, the Glucose Transporter, Allows Uptake ofd-Ribose*
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It is proposed that there are at least two domains for substrate specificity in PtsG with slightly altered recognition properties and xylose transport is virtually abolished, indicating that the residue is directly involved in determining sugar specificity.
Identification and characterization of the tktB gene encoding a second transketolase in Escherichia coli K-12
TLDR
Results indicated that tktA and tktB were responsible for major and minor activities, respectively, of transketolase in E. coli.
Mechanisms Causing Rapid and Parallel Losses of Ribose Catabolism in Evolving Populations of Escherichia coli B
TLDR
The rapid and parallel evolutionary losses of ribose catabolic function thus involved both an unusually high mutation rate, such that Rbs(-) mutants appeared repeatedly in all populations, and a selective advantage in glucose minimal medium that drove these mutants to fixation.
Genetics and sequence analysis of the pcnB locus, an Escherichia coli gene involved in plasmid copy number control
TLDR
Disruption of the pcnB gene by insertional mutagenesis caused a reduction in growth rate, indicating that PcnB has an important cellular function.
Cloning of genes involved in myo-inositol transport in a Pseudomonas sp
A soil isolate of a Pseudomonas sp. can utilize myo-inositol (MI) as the sole carbon source. In this strain, MI is transported through the membrane by a high-affinity transport system in which a
Structural and functional analyses of the repressor, RbsR, of the ribose operon of Escherichia coli
TLDR
DNA‐binding assays indicate that RbsR binds to a region of perfect dyad symmetry spanning the rbs operon transcriptional start site and that the affinity for the rts operator is reduced by addition of ribose, consistent with ribose being the inducer of the operon.
Molecular interactions in ribose transport: the binding protein module symmetrically associates with the homodimeric membrane transporter
TLDR
The mode of association proposed here for the ribose transport components could be extended to other ABC transporters with similar structural organizations and is consistent with the proposal that the binding protein module interacts symmetrically with homodimeric RbsC.
Identification of the genes in multicopy plasmids affecting ompC and ompF expression in Escherichia coli.
TLDR
The results suggest that ompF and ompC expression is associated with other physiological regulating systems in addition to osmoregulation.
Genetic analysis of a relaxed substrate specificity aromatic ring dioxygenase, toluate 1,2-dioxygenase, encoded by TOL plasmid pWW0 of Pseudomonas putida
SummaryToluate 1,2-dioxygenase is the first enzyme of a meta-cleavage pathway for the oxidative catabolism of benzoate and substituted benzoates to Krebs cycle intermediates that is specified by TOL
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The high-affinity transport-defective mutants were able to utilize D-ribose, indicating that at least a second, low-Affinity transport system for D- ribose is present in Escherichia coli K-12.
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