Molecular cloning and characterization of fengycin synthetase gene fenB from Bacillus subtilis.

@article{Lin1998MolecularCA,
  title={Molecular cloning and characterization of fengycin synthetase gene fenB from Bacillus subtilis.},
  author={G H Lin and C. L. Chen and Johannes Scheng-Ming Tschen and S. S. Tsay and Y S Chang and Shui Teng Liu},
  journal={Journal of bacteriology},
  year={1998},
  volume={180 5},
  pages={1338-41}
}
A fengycin synthetase gene, fenB, has been cloned and sequenced. The protein (FenB) encoded by this gene has a predicted molecular mass of 143.6 kDa. This protein was overexpressed in Escherichia coli and was purified to near homogeneity by affinity chromatography. Experimental results indicated that the recombinant FenB has a substrate specificity toward isoleucine with an optimum temperature of 25 degrees C, an optimum pH of 4.5, a Km value of 922 microM, and a turnover number of 236 s(-1… CONTINUE READING

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