Molecular cloning and characterization of an ML-236B (compactin) biosynthetic gene cluster in Penicillium citrinum

@article{Abe2002MolecularCA,
  title={Molecular cloning and characterization of an ML-236B (compactin) biosynthetic gene cluster in Penicillium citrinum},
  author={Yuki Abe and T. Suzuki and Chiho Ono and Kohji Iwamoto and Masahiko Hosobuchi and Hiroji Yoshikawa},
  journal={Molecular Genetics and Genomics},
  year={2002},
  volume={267},
  pages={636-646}
}
Abstract. Cloning of genes encoding polyketide synthases (PKSs) has allowed us to identify a gene cluster for ML-236B biosynthesis in Penicillium citrinum. Like lovastatin, which is produced by Aspergillus terreus, ML-236B (compactin) inhibits the enzyme 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase. Genomic sequencing and Northern analysis showed that nine predicted genes for ML-236B biosynthesis were located within a 38-kb region and were transcribed when ML-236B was produced. The… 
Functional analysis of mlcR, a regulatory gene for ML-236B (compactin) biosynthesis in Penicillium citrinum
TLDR
The evidence suggests that mlcR may indeed be involved in the transcriptional activation of some of the pathway-specific genes required for ML-236B biosynthesis.
Effect of increased dosage of the ML-236B (compactin) biosynthetic gene cluster on ML-236B production in Penicillium citrinum
TLDR
How the ML-236B-producing strain was improved using a cosmid-mediated recombination technique is described, suggesting that increased dosage of the biosynthetic gene cluster was responsible for the enhanced production of ML- 236B.
Molecular basis of ML-236B production in the high-producing mutant No. 41520 of Penicillium citrinum.
TLDR
Functional analysis revealed that a gene, orf1 next to mlcR, was not involved in the ML-236B biosynthesis, but it was involvement in the transcriptional activation of genes along with the ML -236B gene cluster, which suggested that the increase in ML- 236B production was partly due to increased expression of genes involved inML-236 B biosynthesis.
Cloning and characterization of monacolin K biosynthetic gene cluster from Monascus pilosus.
TLDR
In the present study, a bacterial artificial chromosome (BAC) clone, mps01, was screened from the BAC library constructed from Monascus pilosus BCRC38072 genomic DNA, and the putative monacolin K biosynthetic gene cluster was found within a 42 kb region in the mps 01 clone.
Identification and characterization of Penicilliumcitrinum VeA and LaeA as global regulators for ML-236B production
TLDR
Results indicated that VeA and LaeA dominantly control the biosynthesis of ML-236B, and the enhanced production in the strain S-1567 is attributable to the mutation in laeA.
Identification of the mokH gene encoding transcription factor for the upregulation of monacolin K biosynthesis in Monascus pilosus.
TLDR
Analysis of the RT-PCR products demonstrated that the monacolin K biosynthetic genes in the transformant were expressed to a greater extent than those in the wild-type strain.
Improvement of compactin (ML-236B) production by genetic engineering in compactin high-producing Penicillium citrinum
TLDR
The results indicated that genetic engineering is an effective tool to improve compactin production, even in compactin high producers.
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