Molecular cloning and characterization of aldehyde oxidases in Arabidopsis thaliana.

@article{Sekimoto1998MolecularCA,
  title={Molecular cloning and characterization of aldehyde oxidases in Arabidopsis thaliana.},
  author={Hitoshi Sekimoto and Mitsunori Seo and Naoto Kawakami and Teruya Komano and Sophie Desloire and Sylviane Liotenberg and Annie Marion-Poll and Michel Caboche and Yuji Kamiya and Tomokazu Koshiba},
  journal={Plant & cell physiology},
  year={1998},
  volume={39 4},
  pages={
          433-42
        }
}
Using degenerate primers designed by deduced amino acid sequences of known aldehyde oxidases (AO) from maize and bovine, two independent cDNA fragments were amplified by reverse transcription-polymerase chain reaction (PCR). The two corresponding full-length cDNAs (atAO-1 and atAO-2; 4,484 and 4,228 bp long, respectively) were cloned by screening the Arabidopsis cDNA library followed by rapid amplification of cDNA end-PCR. These cDNAs are highly homologous at both the nucleotide and amino acid… CONTINUE READING
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