Molecular cloning and characterization of a novel lactate dehydrogenase gene from Clonorchis sinensis

@article{Yang2005MolecularCA,
  title={Molecular cloning and characterization of a novel lactate dehydrogenase gene from Clonorchis sinensis},
  author={Guang Yang and Chunxia Jing and Peixian Zhu and Xuchu Hu and Jin Xu and Zhongdao Wu and Xinbing Yu},
  journal={Parasitology Research},
  year={2005},
  volume={99},
  pages={55-64}
}
From a Clonorchis sinensis adult worm cDNA library, we isolated a cDNA clone encoding a novel lactate dehydrogenase (LDH) gene which encoded a putative protein with a predicted molecular weight of 35.6 kDa. The optimum pH and temperature for the enzyme were 7.5 and 50°C in the pyruvate reduction while 11 and 80°C in the lactate oxidation reaction, respectively. CsLDH showed no substrate inhibition by high lactate and NAD+ concentration, and the optimal pyruvate and optimal NADH concentrations… CONTINUE READING

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