Molecular cloning and characterization of a novel casein kinase II substrate, HASPP28, from rat brain.

  title={Molecular cloning and characterization of a novel casein kinase II substrate, HASPP28, from rat brain.},
  author={L. Shen and K. Huang and H. Chen and F. Huang},
  journal={Archives of biochemistry and biophysics},
  volume={327 1},
HASPP28 (heat- and acid-stable phosphoprotein of 28 kDa) has been purified to near homogeneity from the acid-stable protein fraction of rat brain extract. Based on the N-terminal 40 amino acid sequence, a pair of highly degenerate primers was used to generate a 107-bp probe from rat brain RNA by RT-PCR. From the rat brain lambda gt11 library, this probe identified two positive clones that together provided a cDNA of 837 bp with an open reading frame of 546 bp. This cDNA was extended by 3'RACE… Expand
Casein kinase 2 as a potentially important enzyme in the nervous system
  • P. Blanquet
  • Medicine, Biology
  • Progress in Neurobiology
  • 2000
Some data are suggestive that CK2 might play a role in processes underlying progressive disorders due to Alzheimer's disease, ischemia, chronic alcohol exposure or immunodeficiency virus HIV, as well as the possible neurophysiological functions of this enzyme. Expand
Protein kinase CK2 ("casein kinase-2") and its implication in cell division and proliferation.
A number of functional features of the beta-subunit which could cooperate to the modulation of CK2 targeting/activity will be discussed. Expand
Identification of Candidate Casein Kinase 2 Substrates in Mitosis by Quantitative Phosphoproteomics
It is demonstrated that overexpression of CK2 in HeLa cells affects proper chromosome condensation and CX-4945 and quantitative phosphoproteomics are utilized to inhibit CK2 activity in mitotically arrested He La cells and determine candidate CK2 substrates. Expand
HUPO BPP pilot study: A proteomics analysis of the mouse brain of different developmental stages
Western blotting analysis demonstrated that enolase is a protein up‐regulated in the mouse brain from embryonic stage to adult stage and these data are helpful for understanding the proteome changes in the development of the Mouse brain. Expand
The putative kinase substrate MUSE7 negatively impacts the accumulation of NLR proteins
A role for MUSE7 is demonstrated in modulating plant immune responses through negatively affecting NLR accumulation, and will benefit future studies of Muse7 homologs in other species. Expand
Differentially expressed genes in hippocampal cell cultures in response to an excitotoxic insult by quinolinic acid.
Evaluating the alterations in gene expression in a primary hippocampal cell culture after exposure to quinolinic acid provides emphasis to the participation of proteolysis and protease inhibition in neurodegenerative processes. Expand
Increased expression of platelet-derived growth factor associated protein-1 is associated with PDGF-B mediated glioma progression.
The findings suggest that the expression of PDAP-1 is associated with disease malignancy, and its inhibition reduced the proliferation of malignant glioma cells through down-regulation of PDGF-B/Akt/PDK1 signaling. Expand
Pdap1 protects mature B lymphocytes from stress-induced cell death and promotes antibody gene diversification
  • V. Delgado-Benito, Maria Berruezo-Llacuna, +10 authors Michela Di Virgilio
  • Biology
  • 2020
PDGFA-associated protein 1 (Pdap1) protects mature B cells against chronic ISR activation and ensures efficient antibody diversification by promoting their survival and optimal function. Expand
PDGFA-associated protein 1 protects mature B lymphocytes from stress-induced cell death and promotes antibody gene diversification
Pdap1 protects mature B cells against chronic induction of the integrated stress response and promotes both their survival and antibody gene diversification by CSR and SHM. Expand
Regulating the plant innate immune system : the roles of three Arabidopsis MUSE proteins
......................................................................................................................................... ii PrefaceExpand