Molecular cloning and characterization of a gene encoding pea cytosolic ascorbate peroxidase.

@article{Mittler1992MolecularCA,
  title={Molecular cloning and characterization of a gene encoding pea cytosolic ascorbate peroxidase.},
  author={Ron Mittler and Barbara A. Zilinskas},
  journal={The Journal of biological chemistry},
  year={1992},
  volume={267 30},
  pages={21802-7}
}
A gene encoding cytosolic ascorbate peroxidase (ApxI) from pea (Pisum sativum L.) was isolated and its nucleotide sequence determined. By homologous alignment between the ApxI cDNA (Mittler, R., and Zilinskas, B. (1991) FEBS Lett. 289, 257-259) and the genomic clone, positions of introns and exons were determined. The isolated ApxI gene was found to contain 9 introns, the first of which was located within the 5'-untranslated region of the mRNA. Southern blot analysis of pea genomic DNA suggests… CONTINUE READING

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