Molecular cloning and characterization of a steroidogenic enzyme, 17β-hydroxysteroid dehydrogenase type 14, from the stony coral Euphyllia ancora (Cnidaria, Anthozoa).

  title={Molecular cloning and characterization of a steroidogenic enzyme, 17$\beta$-hydroxysteroid dehydrogenase type 14, from the stony coral Euphyllia ancora (Cnidaria, Anthozoa).},
  author={Shinya Shikina and Yi-Jou Chung and Yi-Ling Chiu and Yida Huang and Yan-Horn Lee and Ching-Fong Chang},
  journal={General and comparative endocrinology},
Sea Anemones Responding to Sex Hormones, Oxybenzone, and Benzyl Butyl Phthalate: Transcriptional Profiling and in Silico Modelling Provide Clues to Decipher Endocrine Disruption in Cnidarians
This suite of GOIs are candidate biomarkers for detecting transcriptional changes in steroidogenesis, gametogenesis, sterol transport, and Hedgehog signaling, and detection of disruptions in these pathways offers new insight into endocrine disruption in cnidarians.
Discovery of a receptor guanylate cyclase expressed in the sperm flagella of stony corals
This study is the first to perform molecular characterization of r GCs in cnidarians and provides evidence for the possible involvement of rGCs in the reproduction of basal metazoans and suggests that GC-A may be involved in the regulation of sperm activity and/or functions in corals.
De novo transcriptome assembly from the gonads of a scleractinian coral, Euphyllia ancora: molecular mechanisms underlying scleractinian gametogenesis
This study is the first to create a gonadal transcriptome assembly from any scleractinian, and will be a useful reference for future development of sex-specific and/or stage-specific germ cell markers that can be used in coral aquaculture and ecological studies.
Molecular cloning and characterization of proliferating cell nuclear antigen; its potential involvement in germ cell development of stony corals
This study is the first to show the expression profile of the PCNA protein and its subcellular localization in coral germ-cell development and suggests that PCNA plays an important role in both the mitotic and meiotic processes of the coral germ cells.
Oocytes express an endogenous red fluorescent protein in a stony coral, Euphyllia ancora: a potential involvement in coral oogenesis
The present study provides not only the first evidence for the potential involvement of FPs in coral oogenesis but also an insight into a cellular strategy underlying coral sexual reproduction.
Does estrogen regulate vitellogenin synthesis in corals?


17Beta-hydroxysteroid dehydrogenase (17beta-HSD) in scleractinian corals and zooxanthellae.
The data confirm that corals and zooxanthellae contain 17beta-HSD and are consistent with the presence of more than one isoform of the enzyme, which was the most inhibitory of the steroids and phytoestrogens tested.
Structural and biochemical characterization of human orphan DHRS10 reveals a novel cytosolic enzyme with steroid dehydrogenase activity.
Evidence is presented that the previously functionally uncharacterized product of the human DHRS10 gene is endowed with 17beta-HSD (17beta-hydroxysteroid dehydrogenase) activity, which suggests a role for DHRS 10 in the local inactivation of steroids in the central nervous system and placenta.
Steroid metabolism in cnidarians: Insights from Nematostella vectensis
Zebrafish 17beta-hydroxysteroid dehydrogenases: An evolutionary perspective
Expression patterns of 17β-hydroxysteroid dehydrogenase 14 in human tissues.
Investigation of sex steroid conversional activity in HSD17B14 overexpressing HEK293 and MCF10A cells and immunohistochemical staining patterns of 17βHSD14 with the enzyme being primarily expressed in glandular epithelial tissue reveal an enzyme with possible implications in the secretion or conversion of externally derived compounds.
Structure and function of human 17beta-hydroxysteroid dehydrogenases.
Broad and overlapping substrate specificities with enzymes involved in lipid metabolism suggest interactions of several 17beta-HSDs with other metabolic pathways, of particular importance in cancer, metabolic diseases, neurodegeneration and possibly immunity.
Testosterone metabolism in the estuarine mysid neomysis integer (Crustacea; Mysidacea): identification of testosterone metabolites and endogenous vertebrate-type steroids.
Alterations in steroid hormone metabolism may be used as a new biomarker for the effects of endocrine disruptors in invertebrates and vertebrate species.